HCA ImagAmp kits for amplified detection of fluorescent signal in high content screening (HCS) applications.
Based on 30 μL per well of Fluorophore Amplification Reagent Working Solution
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HCA ImagAmp 488 kits provide signal amplification for high content screening and analysis assays, delivering enhanced sensitivity and improved dynamic range. It is ideal for assays with very low signal due to low abundance of the analyte or weakly associated primary antibodies. The HCA ImagAmp protocol results in covalent deposition of multiple fluorophores proximal to the analyte during a simple 10-minute incubation step.
|Product Brand Name||HCA ImagAmp|
|Shipping Condition||Blue Ice|
|Unit Size||1 microplate|
Now you can measure what was previously undetectable in high content screening (HCS) immunofluorescence assays. HCA ImagAmp? kits from PerkinElmer leverage our proprietary Tyramide Signal Amplification? (TSA) technology to deliver unprecedented sensitivity without compromising your image resolution.
HCA ImagAmp is based on Tyramide Signal Amplification (TSA?), a technology from PerkinElmer that generally provides 10 to 100-fold of improved sensitivity while allowing reduced consumption of primary antibodies. TSA uses horseradish peroxidase (HRP) to catalyze covalent labeling directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited fluorophores can then be detected immediately by standard fluorescence visualization techniques, with significant enhancement of the signal